Iceberg lettuce cultivated in different systems of planting and sources of fertilizer / Alface americana cultivada em diferentes sistemas de plantio e fontes de fertilizantes

Organic fertilization is a cheaper and highly effective option for profitability and consequent improvement of the soil's physical, chemical, and biological structure. Thus, the objective of this work was to evaluate different types of fertilization: organic (poultry shed litter), mineral, and leaf path on yield parameters of lettuce grown in various types of planting. The treatments consisted of using two planting systems (P1 - Line and P2 - quincunxes) and mineral and organic fertilizers (A1 - mineral fertilization; A2 - mineral fertilization + leaf fertilization; A3 - organic fertilization with poultry shed litter and A4 - fertilization organic + mineral). The experimental units consisted of 36 and 52 plants, respectively, for treatments P1 and P2, and all central plants of the experimental unit were evaluated. Heart height, fresh mass, and leaf number were observed. The mineral and mineral + leaf treatments did not differentiate, either in line or in quincunxes. The treatment that stood out about the analyzed variables was the organic fertilization and quincunxes planting system, reflecting a more significant number of lettuce plants and better use of the area.

A adubação orgânica é uma opção mais barata e de grande eficácia em relação à rentabilidade e consequente melhoria da estrutura física, química e biológica do solo. Desta forma, o objetivo deste trabalho foi avaliar diferentes tipos de adubação: orgânica (cama de frango), mineral e via foliar sobre parâmetros de produtividade de alface cultivada em diferentes tipos de plantio. Os tratamentos consistiram na utilização de dois sistemas de plantio (P1 - Linha e P2 - Quincôncio) e adubações minerais e orgânicas (A1 - adubação mineral; A2 - adubação mineral + adubação foliar; A3 - adubação orgânica com cama de aviário e A4 - adubação orgânica + mineral). As unidades experimentais foram compostas por 36 e 52 plantas, respectivamente, para os tratamentos em linha e em quincôncio, sendo avaliadas todas as plantas centrais da unidade experimental. Foram observados a altura do coração, massa fresca e número de folhas. Os tratamentos mineral e mineral + foliar não diferenciaram entre si, tanto em linha quanto em quincôncio. O tratamento que se destacou em relação às variáveis analisadas foi aquele baseado na adubação orgânica e sistema de plantio em quincôncio, refletindo em maior número de pés de alface e melhor aproveitamento da área.

What is the optimal fertigation start time and frequency in lettuce seedlings?

Although lettuce is one of the most important vegetable crops cultivated in Brazil, producers conduct seedling production empirically, as there are no published reports on the optimal start time and management strategy for seedling fertigation. The present aimed to assess the influence of fertigation management on the growth, physiological aspects and nutritional status of lettuce seedlings and to determine the optimal fertigation start time and frequency. Two experiments were conducted, each with a randomized block design and six repetitions. The first consisted of six treatments, namely six fertigation start times at 0, 3, 6, 9, 12, and 15 d after emergence (DAE), and the second consisted of five treatments, representing different application frequencies at 3, 4, 5, 6, and 7 d intervals. The assessment of nutrient accumulation levels and biometric and physiological characteristics of the seedlings were performed after transplanting. Fertigation start times significantly affected 14 of the 18 variables assessed, particularly the number of leaves, shoot dry weight, leaf area, initial chlorophyll fluorescence, and P, K, Ca, Mg, and S accumulation. The best results for ten variables were obtained when fertigation began at emergence, with values 17.77 - 35.63% higher than those at fertigation onset at 15 DAE. Application frequency only influenced chlorophyll content and N, P, K, and S accumulation, with optimal results obtained at 3 - 6 d intervals. Beginning fertigation at plant emergence favors dry weight production, nutrition and photosynthesis and shortens the production time of lettuce seedlings. The optimal start time for lettuce seedling fertigation is at emergence, with application performed every 6 d.

Contaminação parasitária em hortaliças comercializadas no município de Jataí-GO / Parasitic contamination in vegetables commercialized in the city of Jataí-GO

Introdução: a prevalência de doenças parasitárias transmitidas por alimentos tem apresentado um aumento significativo em nível mundial, destacando principalmente as hortaliças, consumidas in natura, em razão da sociedade moderna que procura hábitos de vida mais saudáveis. Objetivo:avaliar a contaminação de hortaliças comercializadas no município de Jataí, GO, por enteroparasitos. Metodologia: foi realizada uma pesquisa experimental para detecção de estruturas parasitárias em hortaliças de cultivo tradicional e hidropônico, obtidas de nove estabelecimentos: três supermercados, três hortas e três feiras livres. Foram obtidas 54 amostras de alface (Lactuca sativa), 54 de rúcula (Chicarium sp.) e 18 de agrião (Nasturtiumofficinale). As amostras foram lavadas com água destilada e solução Tween 80 e submetidas as técnicas de sedimentação espontânea e por centrifugação. Foi realizado um mapeamento da área de estudo para identificação da localização dos estabelecimentos. Resultados: a positividade para estruturas parasitárias foi de 94,4% (119/126). A alface apresentou maior prevalência de parasitos 96,2% (52/54). Foi observada diferença estatística significativa ao comparar o emprego das duas técnicas (p < 0,05). Ashortaliças submetidas a técnica de sedimentação espontânea apresentaram maior positividade 115/126 (91,3%). Não foi observada diferença estatística significante quanto ao tipo de cultivo, porém, houve forte associação em relação ao local de cultivo e contaminação das hortaliças (OR: 3,625). Verificou-se maior percentual de positividade nos estabelecimentos localizados próximos aos rios. Conclusão: a positividade de enteroparasitos detectada nas hortaliças foi alta, ressaltando a necessidade de implementar medidas que visam a conscientização da população, em relação às práticas de higienização das hortaliças antes do consumo.

Introduction: the prevalence of parasitic diseases transmitted by food has shown a significant increase worldwide, especially vegetables, consumed in natura, due to modern society that seeks healthier lifestyles. Objective: to evaluate the contamination of vegetables commercialized in the city of Jataí, GO, by enteroparasites. Methodology: an experimental research was carried out to detect parasitic structures in vegetables of traditional and hydroponic cultivation, obtained from nine establishments: three supermarkets, three vegetable gardens and three street markets. Fifty four samples of lettuce (Lactuca sativa), 54 of arugula (Chicarium sp.) and 18 of watercress (Nasturtium officinale) were obtained. The samples were washed with distilled water and Tween 80 solution and submitted to spontaneous sedimentation and centrifugation techniques. A mapping of the study area was carried out to identify the location of the establishments. Results: positivity for parasitic structures was 94.4% (119/126). Lettuce had a higher prevalence of parasites 96.2% (52/54). A statistically significant difference was observed when comparing the use of the two techniques (p < 0.05). Vegetables submitted to the spontaneous sedimentation technique showed higher positivity 115/126 (91.3%). There was no statistically significant difference regarding the type of cultivation, however, there was a strong association in relation to the place of cultivation and contamination of vegetables (OR: 3.625). There was a higher percentage of positivity in establishments located near the rivers. Conclusion: the positivity of intestinal parasites detected in vegetables was high, highlighting the need to implement measures aimed at raising awareness of the population regarding the practices of cleaning vegetables before consumption.

Doses of sulfur at topdressing and organic compost supply at planting in the production, quality and content of macronutrients in lettuce seeds

The purpose of this study was to evaluate doses of sulfur applied at topdressing, with and without organic compost supply at planting, in the production, physiological quality and content of macronutrients in lettuce seeds. Ten treatments were evaluated in a 5x2 factorial scheme (five sulfur doses at topdressing x with and without application of organic compost at planting). The experimental design was a randomized block design, with four replicates. The sulfur doses applied at topdressing fertilization were 50, 100, 150 and 200 kg ha-1 of S, in addition to the control treatment (dose zero). Regarding the treatments with organic compost, the dose of 70 t ha-1 of compost (wet basis) was supplied at planting. The following characteristics were evaluated: seed production (mass and number of seeds per plant), seed quality (mass of one thousand seeds, first count of the standard germination test, germination percentage, and germination speed index) and content of macronutrients and mineral accumulation. The organic compost supply at planting increased seed production by 43% compared to the treatment without the application of organic compost, while the doses of sulfur did not affect the production of lettuce seeds. The supply of organic compost increased the accumulation of a great part of the macronutrients, except for the accumulation of calcium. The lettuce seeds quality was not affected by both the main treatments, the sulfur doses at topdressing and the organic compost supply at planting. The descending order of nutrients accumulated in the lettuce seeds was nitrogen> potassium> phosphorus> magnesium> calcium> sulfur.

Reaction of biofortified lettuce genotypes to different strains of Xanthomonas campestris pv. vitians

Lettuce bacterial leaf spot caused by Xanthomonas campestris pv. vitians is an aggressive disease that is difficult to control. So far there are no reports of the reaction of biofortified lettuce genotypes to different isolates of the bacteria. Thus, the objective was to evaluate the aggressiveness of X. campestris pv. vitians, as well as the reaction of biofortified lettuce genotypes to bacterial spot. Two experiments were performed in two distinct seasons (winter and summer), in greenhouse at the Vegetable Experimental Station of the Federal University of Uberlândia (UFU). The experimental design in both experiments was a randomized block design, in a factor scheme of 5 × 4 (five genotypes and four strains), with four repetitions. Were evaluated the severity and the area under the disease progress curve. In general, the biofortified lettuce 'Uberlândia 10000' was more resistant to most bacterial strains in the summer cultivation, and in the winter period UFU 'Crespa 206'. The commercial cultivar Robusta was the most susceptible to the strains during both seasons. The UFU E125 strain was the most aggressive for most genotypes in both seasons.

Detection of enteropathogens and research of pesticide residues in Lactuca sativa from traditional and agroecological fairs / Detecção de enteropatógenos e pesquisa de resíduos de agrotóxicos em Lactuca sativa de feiras tradicionais e agroecológicas

Agroecological production represents a new reality in vegetable farming. Thus, the study aimed to search for the presence of pesticides in agroecological samples and microbiological and parasitological contamination in Lactuca sativa L. of agroecological and conventional fairs in Alagoas. Thirty-two samples were collected, a hygienicsanitary checklist was performed, along with coliform, Salmonella spp., Staphylococcus aureus research and Gas Chromatography with Mass Spectrometer (GC-MS) for pesticide research; using two methodologies for parasitological studies. Thirty-two samples were analyzed, with a sanitary adequacy level at 45% and 38% for fairs (agroecological and conventional) respectively, with three samples (9.4%) in total, being adequate to the microbiological pattern according to RDC no. 12/2001. Among the 23 samples which were tested positive for parasites, 69.5% originated from samples extracted at conventional fairs, and 53% of every positive samples presented Entamoeba coli as the main contaminant. All together, no traces of deltamethrin were detected, but in two of the samples a peak retention for diphenoconazole was detected. In conclusion, the fairs represent an adequate environment for human pathogens and indicators. Therefore greater hygiene training of vegetable traders and consumers in the state of Alagoas is required, especially in conventional fairs, which presented more critical microbiological and parasitological indexes comparing to fairs with agroecological products; however, the presence of difenoconazole in two samples of agricultural fairs suggests accidental contamination or indiscriminate use of this chemical in the production of Lactuca sativa, however, products with agroecological origin represents a better choice for the consumers.

A produção agroecológica representa uma nova realidade no cultivo das hortaliças. Assim, o estudo teve como objetivo verificar a presença de agrotóxicos em amostras agroecológicas e analisar a contaminação microbiológico e parasitológico em Lactuca sativa L. de feiras agroecológicas e convencionais de Alagoas. Foram coletadas 32 amostras, realizando checklist higiênico-sanitário, análises de coliformes, Salmonella spp., Staphylococcus aureus e Cromatografia Gasosa com Espectrômetro de Massas (GC-MS) para pesquisa de agrotóxicos; utilizando-se duas metodologias para estudos parasitológicos. Foram analisadas 32 amostras, com nível de adequação sanitária de 45% e 38% para feiras (agroecológicas e convencionais) respectivamente, sendo três amostras no total (9,4%) adequadas a padrão microbiológico segundo RDC n° 12//2001; Dentre as 23 amostras positivas para parasitos, 69,5% teve origem em amostras de feiras convencionais, e 53% de todas as amostras positivas apresentaram Entamoeba coli como principal contaminante, juntamente, não foi detectado traços de deltametrina, porem foi visualizado em duas amostras, pico de retenção para padrão de difenoconazol. Concluindo-se que as feiras representam um ambiente adequado para patógenos humanos e indicadores, necessitando de uma maior higienização e treinamento dos comerciantes e consumidores de hortaliças do estado de Alagoas, principalmente em feiras convencionais, que apresentaram índices microbiológicos e parasitológicos mais críticos em relação a feiras com produtos agroecológicos; mas, a presença de difenoconazole em duas amostras de feiras agroecológicas sugerem contaminação acidental ou uso indiscriminado deste químico em produção da Lactuca sativa L., todavia os produtos de origem agroecológica representam uma melhor escolha para o consumidor.

Chemical composition and phytotoxicity of essential oils of Croton doctoris S. Moore (Euphorbiaceae) / Composição química e fitotoxicidade dos óleos essenciais de Croton doctoris S. Moore (Euphorbiaceae)

Essential oils from the stems and leaves of Croton doctoris were analyzed by gas chromatography and mass spectrometry, resulting in 22 identified compounds. The effects of these essential oils on the germination, root and shoot growth, total chlorophyll content, potential root respiration, peroxidase activity, catalase, superoxide dismutase, and mitotic index in lettuce and onion were determined. Antioxidant, antimicrobial, and cytotoxic activity were also investigated. The results revealed that the stem oil consisted of 15 compounds, of which caryophyllene oxide (24.5%) and E-caryophyllene (13.3%) were the major constituents. The leaf oil contained E-caryophyllene (39.6%) and α-humulene (13.2%) as major compounds. The oils inhibited the germination and growth of lettuce and onion seedlings and reduced chlorophyll content, root respiration, and cell division. They also caused oxidative stress, indicated by the increased activity of the evaluated antioxidant enzymes. These abnormal physiological processes contributed to the inhibition of plant growth. The most pronounced phytotoxic effects were observed in the stem oil. The cytotoxicity tests indicated that leaf oil was more active than stem oil, resulting from the presence of biologically active sesquiterpenes that inhibit the growth of cancer cells.

Os óleos essenciais do caule e da folha de Croton doctoris foram analisados por cromatografia gasosa (GC) e espectrometria de massa (GC-MS) resultando em 22 compostos identificados. Os efeitos dos óleos essenciais na germinação, crescimento de raízes e parte aérea, teor total de clorofila, respiração radicular, atividade de peroxidase, catalase e superóxido de dimetase e índice mitótico foram determinados em alface e cebola. Atividade antioxidante, antimicrobiana e citotóxica também foram investigadas. Os resultados revelaram que o óleo do caule é constituído por 15 compostos, dos quais os principais são o óxido de cariofileno (24,5%) e E-cariofileno (13,3%). O óleo foliar apresentou E-cariofileno (39,6%) seguido de α-humuleno (13,2%) como compostos majoritários. Os óleos inibiram a germinação e o crescimento das plântulas de alface e cebola e reduziram o conteúdo de clorofila, a respiração radicular e a divisão celular. Eles também causaram estresse oxidativo, indicado pelo aumento da atividade das enzimas antioxidantes avaliadas. Esses processos fisiológicos anormais contribuem para a inibição do crescimento das plantas. Os efeitos fitotóxicos mais pronunciados foram observados no óleo do caule. Nos testes de citotoxicidade observou-se que o óleo das folhas foi mais ativo, resultante da presença de sesquiterpenos biologicamente ativos que atuam inibindo o crescimento das células cancerígenas.

A Physiological behavior and tolerance of Lactuca sativa to lead nitrate and silver nitrate heavy metals / Comportamento fisiológico e tolerância de Lactuca sativa aos metais pesados nitrato de chumbo e nitrato de prata

This study goal to evaluate the effects of different concentrations of lead (Pb) and silver (Ag) on germination, initial growth and anatomical alterations of Lactuca sativa L. Plants use various mechanisms to reduce the impacts caused by anthropic action, such as xenobiotic elements of soils and water contaminated by heavy metals. These metals were supplied as lead nitrate and silver nitrate and the following treatments were established: control for both metals, maximum dose of heavy metals, for arable soils, allowed by the National Council of the Environment (Ag = 25 mg. Kg-1, Pb = 180 mg. Kg-1), double (Ag = 50 mg. Kg-1, Pb = 360 mg. Kg-1) and triple (Ag = 75 mg. Kg-1, Pb = 540 mg. Kg -1) of this dosage. Vigor and germination tests of the seeds and possible anatomical changes in the leaves and roots of lettuce plants were performed. The species showed a high capacity to germinate under Pb and Ag stress, and the germination was never completely inhibited; however, the germination decreased with increasing Pb concentrations, but not under Ag stress. The use of increasing doses of metals reduced seed vigor and increased chlorophyll content. An increase in biomass was also observed in plants from treatments submitted to Pb. The phytotoxic effects of metals were more pronounced at 15 days after sowing. Anatomically, L. sativa was influenced by metal concentrations, and had a reduction of up to 79.9% in root epidermis thickness at the highest Pb concentration, although some structures did not suffer significant changes. The results suggest that L. sativa presents tolerance to high concentrations of heavy metals, showing possible mechanisms to overcome the stress caused by these metals. In this research lettuce possibly used the mechanism of exclusion of metals retaining Pb and Ag in the roots preserving the photosynthetic apparatus in the aerial part of the plants. In general, the chemical element Pb was more toxic than Ag, in these experimental conditions.

Este estudo teve como objetivo avaliar os efeitos de diferentes concentrações de chumbo (Pb) e prata (Ag) na germinação, crescimento inicial e alterações anatômicas de Lactuca sativa L. As plantas utilizam vários mecanismos para reduzir os impactos causados pela ação antrópica, como elementos xenobióticos de solos e água contaminada por metais pesados. Esses metais foram fornecidos como nitrato de chumbo e nitrato de prata e foram estabelecidos os seguintes tratamentos: controle para ambos os metais, dose máxima de metais pesados, para solos cultiváveis, permitida pelo Conselho Nacional do Meio Ambiente (Ag = 25mg.Kg-1, Pb = 180mg.Kg-1), dobro (Ag = 50mg.Kg-1, Pb = 360mg.Kg-1) e triplo (Ag = 75mg.Kg-1, Pb = 540 mg.Kg -1) desta dosagem. Foram realizados testes de vigor e germinação das sementes e possíveis alterações anatômicas nas folhas e raízes das plantas de alface. A espécie apresentou alta capacidade de germinar sob estresse de Ag e Pb, e a germinação nunca foi completamente inibida; entretanto, a germinação diminuiu com o aumento das concentrações de Pb, mas não sob estresse de Ag. O uso de doses crescentes dos metais, reduziu o vigor das sementes e aumentou o teor de clorofila. Também foi observado aumento da biomassa nas plantas a partir dos tratamentos submetidos ao Pb. Os efeitos fitotóxicos dos metais foram mais acentuados aos 15 dias após a semeadura. Anatomicamente, L. sativa foi influenciada pelas concentrações de metais, e teve uma redução de até 79,9% na espessura da epiderme radicular na maior concentração de Pb, embora algumas estruturas não tenham sofrido alterações significativas. Os resultados sugerem que L. sativa apresenta tolerância a altas concentrações de metais pesados, mostrando possíveis mecanismos para superar o estresse causado por esses metais. Nesta pesquisa a alface possivelmente utilizou o mecanismo de exclusão de metais retendo Pb e Ag nas raízes preservando o aparato fotossintético na parte aérea das plantas. De forma geral o elemento químico Pb se mostrou mais tóxico que Ag, nestas condições experimentais.

Phytotoxicity of plant extracts of Vismia japurensis cultivated in vivo and in vitro / Fitotoxicidade de extratos de plantas de Vismia japurensis cultivadas in vivo e in vitro

Plants that produce secondary metabolites with allelopathic activity or phytotoxicity can be biotechnologically important, serving as sources of allelochemicals, and thus contributing to the agroindustrial sector. Vismia japurensis (Hypericaceae) is an Amazonian species that grows in clumps called vismiais, from which most other plants are absent. Accordingly, the objective of this study was to identify possible phytotoxicity effects of hexane and methanol extracts of Vismia japurensis leaves and branches in vivo and from seedlings grown in vitro on Lactuca sativa. In addition, fresh and dry leaves were assayed by the sandwich method in order to determine their ability to release allelochemicals. The hexanic extract from in vitro seedlings reduced germination by 10%, while the methanol extract produced a 16% reduction in germination speed. Root growth of Lactuca sativa was inhibited by 64.7% when subjected to hexane leaf extract, by 39.3% under the influence of hexane branch extract, and by 96.09% for in vitro seedling hexanic extract. When analysed by thin layer chromatography and 1H nuclear magnetic resonance, extracts showed evidence of terpenes, anthraquinones and flavonoids, with greater intensity of signals in the aromatic region of in vitro seedling hexanic extract. Clearly, Vismia japurensis has a high biotechnological potential in terms of the production of substances of low polarity with capacity to interfere in plant development.

Plantas que produzem metabólitos secundários com atividade alelopática ou fitotóxica podem ser biotecnologicamente importantes, servindo como fontes de aleloquímicos e, assim, contribuindo para o setor agroindustrial. Vismia japurensis (Hypericaceae) é uma espécie amazônica que cresce em grupos, formando vismiais. Assim, o objetivo deste estudo foi identificar possíveis efeitos fitotóxicos de extratos hexânicos e metanólicos de folhas e ramos de Vismia japurensis in vivo e de plântulas cultivadas in vitro sobre Lactuca sativa. Além disso, folhas frescas e secas foram analisadas pelo método sanduíche, a fim de determinar sua capacidade de liberação de aleloquímicos. O extrato hexânico de plântulas in vitro reduziu a germinação em 10% e o extrato metanólico promoveu uma redução de 16% na velocidade de germinação. O crescimento radicular de Lactuca sativa foi inibido em 64,7% quando submetido ao extrato hexânico das folhas, em 39,3% sob influência do extrato hexânico dos galhos e em 96,09% para o extrato de hexânico das plântulas in vitro. Quando analisados por cromatografia em camada delgada e ressonância magnética nuclear de 1H, os extratos mostraram evidências de terpenos, antraquinonas e flavonoides, com maior intensidade de sinais na região aromática do extrato hexânico das plântulas in vitro. Assim, Vismia japurensis possui elevado potencial biotecnológico em termos de produção de substâncias de baixa polaridade com capacidade de interferência no desenvolvimento de plantas.

A Physiological behavior and tolerance of Lactuca sativa to lead nitrate and silver nitrate heavy metals / Comportamento fisiológico e tolerância de Lactuca sativa aos metais pesados nitrato de chumbo e nitrato de prata

This study goal to evaluate the effects of different concentrations of lead (Pb) and silver (Ag) on germination, initial growth and anatomical alterations of Lactuca sativa L. Plants use various mechanisms to reduce the impacts caused by anthropic action, such as xenobiotic elements of soils and water contaminated by heavy metals. These metals were supplied as lead nitrate and silver nitrate and the following treatments were established: control for both metals, maximum dose of heavy metals, for arable soils, allowed by the National Council of the Environment (Ag = 25 mg. Kg-¹, Pb = 180 mg. Kg-¹), double (Ag = 50 mg. Kg-¹, Pb = 360 mg. Kg-¹) and triple (Ag = 75 mg. Kg-¹, Pb = 540 mg. Kg-¹) of this dosage. Vigor and germination tests of the seeds and possible anatomical changes in the leaves and roots of lettuce plants were performed. The species showed a high capacity to germinate under Pb and Ag stress, and the germination was never completely inhibited; however, the germination decreased with increasing Pb concentrations, but not under Ag stress. The use of increasing doses of metals reduced seed vigor and increased chlorophyll content. An increase in biomass was also observed in plants from treatments submitted to Pb. The phytotoxic effects of metals were more pronounced at 15 days after sowing. Anatomically, L. sativa was influenced by metal concentrations, and had a reduction of up to 79.9% in root epidermis thickness at the highest Pb concentration, although some structures did not suffer significant changes. The results suggest that L. sativa presents tolerance to high concentrations of heavy metals, showing possible mechanisms to overcome the stress caused by these metals. In this research lettuce possibly used the mechanism of exclusion of metals retaining Pb and Ag in the roots preserving the photosynthetic apparatus in the aerial part of the plants. In general, the chemical element Pb was more toxic than Ag, in these experimental conditions.

Este estudo teve como objetivo avaliar os efeitos de diferentes concentrações de chumbo (Pb) e prata (Ag) na germinação, crescimento inicial e alterações anatômicas de Lactuca sativa L. As plantas utilizam vários mecanismos para reduzir os impactos causados pela ação antrópica, como elementos xenobióticos de solos e água contaminada por metais pesados. Esses metais foram fornecidos como nitrato de chumbo e nitrato de prata e foram estabelecidos os seguintes tratamentos:controle para ambos os metais, dose máxima de metais pesados, para solos cultiváveis, permitida pelo Conselho Nacional do Meio Ambiente (Ag = 25mg.Kg-¹, Pb = 180mg.Kg-¹), dobro (Ag = 50mg.Kg-¹, Pb = 360mg.Kg-¹) e triplo (Ag = 75mg.Kg-¹, Pb = 540 mg.Kg-¹) desta dosagem. Foram realizados testes de vigor e germinação das sementes e possíveis alterações anatômicas nas folhas e raízes das plantas de alface. A espécie apresentou alta capacidade de germinar sob estresse de Ag e Pb, e a germinação nunca foi completamente inibida; entretanto, a germinação diminuiu com o aumento das concentrações de Pb, mas não sob estresse de Ag. O uso de doses crescentes dos metais, reduziu o vigor das sementes e aumentou o teor de clorofila. Também foi observado aumento da biomassa nas plantas a partir dos tratamentos submetidos ao Pb. Os efeitos fitotóxicos dos metais foram mais acentuados aos 15 dias após a semeadura. Anatomicamente, L. sativa foi influenciada pelas concentrações de metais, e teve uma redução de até 79,9% na espessura da epiderme radicular na maior concentração de Pb, embora algumas estruturas não tenham sofrido alterações significativas. Os resultados sugerem que L. sativa apresenta tolerância a altas concentrações de metais pesados, mostrando possíveis mecanismos para superar o estresse causado por esses metais. Nesta pesquisa a alface possivelmente utilizou o mecanismo de exclusão de metais retendo Pb e Ag nas raízes preservando o aparato [...].

Detection of enteropathogens and research of pesticide residues in Lactuca sativa from traditional and agroecological fairs / Detecção de enteropatógenos e pesquisa de resíduos de agrotóxicos em Lactuca sativa de feiras tradicionais e agroecológicas

Agroecological production represents a new reality in vegetable farming. Thus, the study aimed to search for the presence of pesticides in agroecological samples and microbiological and parasitological contamination in Lactuca sativa L. of agroecological and conventional fairs in Alagoas. Thirty-two samples were collected, a hygienic sanitary checklist was performed, along with coliform, Salmonella spp., Staphylococcus aureus research and Gas Chromatography with Mass Spectrometer (GC-MS) for pesticide research; using two methodologies for parasitological studies. Thirty-two samples were analyzed, with a sanitary adequacy level at 45% and 38% for fairs (agroecological and conventional) respectively, with three samples (9.4%) in total, being adequate to the microbiological pattern according to RDC no. 12/2001. Among the 23 samples which were tested positive for parasites, 69.5% originated from samples extracted at conventional fairs, and 53% of every positive samples presented Entamoeba coli as the main contaminant. All together, no traces of deltamethrin were detected, but in two of the samples a peak retention for diphenoconazole was detected. In conclusion, the fairs represent an adequate environment for human pathogens and indicators. Therefore greater hygiene training of vegetable traders and consumers in the state of Alagoa sis required, especially in conventional fairs, which presented more critical microbiological and parasitological indexes comparing to fairs with agroecological products; however, the presence of difenoconazole in two samples of agricultural fairs suggests accidental contamination or indiscriminate use of this chemical in the production of Lactuca sativa, however, products with agroecological origin represents a better choice for the consumers.

A produção agroecológica representa uma nova realidade no cultivo das hortaliças. Assim, o estudo teve como objetivo verificar a presença de agrotóxicos em amostras agroecológicas e analisar a contaminação microbiológico e parasitológico em Lactuca sativa L. de feiras agroecológicas e convencionais de Alagoas. Foram coletadas 32 amostras, realizando checklist higiênico-sanitário, análises de coliformes, Salmonella spp., Staphylococcus aureus e Cromatografia Gasosa com Espectrômetro de Massas (GC-MS) para pesquisa de agrotóxicos; utilizando-se duas metodologias para estudos parasitológicos. Foram analisadas 32 amostras, com nível de adequação sanitária de 45% e 38% para feiras (agroecológicas e convencionais) respectivamente, sendo três amostras no total (9,4%) adequadas a padrão microbiológico segundo RDC n° 12//2001; Dentre as 23 amostras positivas para parasitos, 69,5% teve origem em amostras de feiras convencionais, e 53% de todas as amostras positivas apresentaram Entamoeba coli como principal contaminante, juntamente, não foi detectado traços de deltametrina, porem foi visualizado em duas amostras, pico de retenção para padrão de difenoconazol. Concluindo-se que as feiras representam um ambiente adequado para patógenos humanos e indicadores, necessitando de uma maior higienização e treinamento dos comerciantes e consumidores de hortaliças do estado de Alagoas, principalmente em feiras convencionais, que apresentaram índices microbiológicos e parasitológicos mais críticos em relação a feiras com produtos agroecológicos; mas, a presença de difenoconazole em duas amostras de feiras agroecológicas sugerem contaminação acidental ou uso indiscriminado deste químico em produção da Lactuca sativa L., todavia os produtos de origem agroecológica representam uma melhor escolha para o consumidor.

Detection of enteroparasites in foliar vegetables commercialized in street- and supermarkets in Aparecida de Goiânia, Goiás, Brazil / Detecção de enteroparasitas em hortaliças comercializadas em feiras e supermercados em Aparecida de Goiânia, Goiás, Brasil

Foliar vegetables contaminated with fecal residues are an important route of transmission of intestinal parasites to humans. The aim of this study was to evaluate the presence of parasitic forms of protozoa and helminths on lettuces (Lactuca sativa) and collard greens (Brassica oleracea) sold in street- and supermarkets in the city of Aparecida de Goiânia, Goiás, Brazil. A total of 30 samples of each vegetable (15 samples from each supermarkets and street markets) was analyzed. All samples were processed by spontaneous sedimentation method and centrifugal flotation. In 45% of the samples, immature forms of intestinal parasites were identified with 66.7% helminths eggs and 33.3% protozoan cysts or oocysts. Significantly more lettuce samples were contaminated with eggs, cysts or oocyst of at least one parasite than collard green samples (U=216; Z=-3.45; P <0.001). The parasitic forms were identified morphologically up to the family level with eggs of Ancylostomatidae, Strongyloididae, Ascarididae and Taeniidae, or oocysts of Eimeriidae, to the genus with Cystoisospora sp. and Toxocara sp., and to the species level with Cystoisospora canis, Dipylidium caninum and Hymenolepis nana. The presence of these infective agents in lettuce and collard green from both street- and supermarkets highlights the high risk of spreading parasites by eating raw vegetables sold in Aparecida de Goiânia.

Hortaliças contaminadas com resíduos fecais são importantes vias de transmissão de parasitas intestinais ao homem. O objetivo deste estudo foi avaliar a presença de formas parasitárias de protozoários e helmintos em alface (Lactuca sativa) e couve (Brassica oleracea) vendidas em feiras e supermercados na cidade de Aparecida de Goiânia, Goiás, Brasil. Um total de 30 amostras de cada vegetal (15 amostras de supermercados e 15 de feiras livres) foi analisado. Todas as amostras foram processadas pelos métodos de sedimentação espontânea e centrífugo-flutuação. Em 45% das amostras foram identificadas formas imaturas de parasitas intestinais sendo 66,7% ovos de helmintos e 33,3% de cistos ou oocistos de protozoários. Significativamente, mais amostras de alface estavam contaminadas com ovos, cistos ou oocistos de pelo menos um parasita do que as amostras de couve (U = 216; Z = -3,45; P <0,001). As formas parasitárias foram identificadas morfologicamente ao nível de família com ovos de Ancylostomatidae, Strongyloididae, Ascarididae e Taeniidae ou oocistos de Eimeriidae, dos gêneros Cystoisospora sp. e Toxocara sp., e ao nível de espécies com Cystoisospora canis, Dipylidium caninum e Hymenolepis nana. A presença desses agentes infecciosos em alface e couve, provenientes tanto de feiras quanto de supermercados, ressalta o alto risco de veiculação de parasitas pela ingestão de hortaliças cruas comercializadas em Aparecida de Goiânia.

Agronomic and sensory evaluation of lettuce in hydroponic system

Lettuce is the most important leafy vegetable in Brazil. Hydroponic lettuce cultivation has grown due to the viability of harvesting throughout the year. In this context, this study aimed to evaluate the agronomic characteristics, color, and preference of curly lettuce in the NFT hydroponic system. Six lineages (6601-1A, 6601-2L, 7016-6A, 7119-1B, 7223-1A, and 7224-4A) and two commercial cultivars (Brida and Vanda) of lettuce were used, in a completely randomized blocks design with four replications. Shoot length, root length, stem length, number of leaves, stem diameter, plant diameter, shoot fresh mass, root fresh mass, chlorophyll content, instrumental color, and sensory characteristics were evaluated. As for agronomic evaluation, the bolting of 6601-2L lettuce was relevant in different attributes. The Vanda lettuce and the lineage 7016-6A presented best performances for shoot fresh mass (399.44 and 378.63 g, respectively), while the lineages 7119-1B and 6601-2L present the worst performance (279.50 and 273.13 g, respectively). There was variation in chlorophyll content and luminosity, however, the evaluators did not notice differences between lettuces for brightness or green color, as well as for crunchy texture. Lettuces 6601-2L, 7224-4A, 6601-1A, Brida, 7223-1A, and 7119-1B were preferred. The variation among plants may be due to different situations, such as harvesting times and bolting, and a direct relationship between agronomic properties and preference among lettuces has not been established yet.

Efficacy of essential oils in the management of soft rot caused by Pectobacterium aroidearum in lettuce

Lettuce is susceptible to several diseases, especially soft rot caused by bacteria of the genus Pectobacterium. Due to the adaptability of this pathogen and the lack of disease control registered for the crop, the objective of this work was to evaluate the effects of essential oils in the management of soft rot caused by P. aroidearum in lettuce. The study was developed at the Universidade do Estado da Bahia, Juazeiro, BA, Brazil, and the essential oils (EOs) of orange, bergamot, lemongrass, palmarosa, citronella, cloves, tea tree, rosemary, sage, and ginger were used in concentrations of 0.25; 0.5; 0.75 and 1.0% to assess the in vitro growth inhibition of the bacterium. Subsequently, the curative effects of the disease were evaluated by applying the EOs that obtained the best results in vitro in lettuce plants of the susceptible variety "Mônica". The treatments were applied, via spraying, 12 hours after inoculation using the bite method with bacterial suspension. The best in vivo treatment was selected to assess its preventive and curative activity, as well as to find the ideal concentration for reducing epidemiological variables and chromatographic characterization. The EOs of palmarosa, sage, citronella, lemongrass, and cloves (0.25%), and that of sage (0.75%), inhibited bacterial growth in vitro. The EO of salvia showed the best results in vivo, inhibiting the growth of the disease in concentrations of 0.50 and 0.75%, so it was selected for the preventive and curative control tests alone. The preventive treatment was not efficient for the management of soft rot in lettuce, however, from the regression analysis, a concentration of 0.64% of the salvia EO was found as a potential for curative control of this bacteriosis, as it reduces the incidence and severity of the disease. Linalyl acetate and linalool were found in higher concentrations in the chromatographic analysis. These components, probably, conferred the bactericidal capacity of the EO of sage, being potential for the use in the control of P. aroidearum in lettuce.

Technical feasibility of conventional and nonconventional vegetables' intercropping under organic fertilization / Viabilidade técnica do cultivo consorciado de hortaliças convencionais e não convencionais sob fertilização orgânica

This work aimed to evaluate the technical feasibility of lettuce, Indian spinach and taro intercropping under organic fertilizer. The experiment was performed at Água Limpa Farm, belonging to the University of Brasília, located in Brasília - DF, from October 2014 to June 2015. A completely randomized block design with seven treatments and four replications was used. Each experimental plot had 18 m2 (4.5 m x 4.0 m), totaling 28 plots. The following treatments were assessed: lettuce monoculture, Indian spinach monoculture, taro monoculture, lettuce/Indian spinach intercropping, lettuce/taro intercropping, Indian spinach/taro intercropping, and lettuce/Indian spinach/taro intercropping. In all treatments, the following spacings were used: 0.25 x 0.25 m (lettuce), 1.0 x 0.6 m (Indian spinach), and 1.0 x 0.3 m (taro). Crop production was evaluated from sample plants taken from the central part of each plot. Two harvests of lettuce, two of Indian spinach and one of taro were carried out during intercropping. In the first cycle, lettuce highest mean yield values were observed in the lettuce monoculture (3.5 kg m-2) and lettuce/Indian spinach intercropping (3.4 kg m-2). The crop reached the commercial size in both production cycles, except when intercropped with Indian spinach in the second cycle. The highest Indian spinach mean fresh mass was recorded in the lettuce/Indian spinach intercropping (974.7 g plant-1; first harvest) and monoculture (327.5 g plant-1; second harvest). The yield of taro rhizomes was significantly higher when intercropped with lettuce, showing a mean value of 16.8 kg m-2. The intercropping arrangements with lettuce increased yield of nonconventional vegetables. The conventional and nonconventional vegetables' intercropping technique increased species diversity in the area without interfering in the agronomic performance and quality of the crops.

Economic study of the cultivation of kale, coriander, lettuce, and chives in intercropping / Estudo econômico do cultivo da couve, coentro, alface e cebolinha em consórcio

Intercropping of vegetables in a poorly planned manner may not achieve the expected economic results, as it is an activity that requires a great technical and administrative capacity of the producer. This study aimed to analyze the economic feasibility of intercropping kale with coriander, lettuce, and chives in relation to monocultures. The experiment was conducted in the Center for Agri-food Science and Technology, Federal University of Campina Grande, in the municipality of Pombal, PB in the period from June 2014 to July 2015. Eleven treatments were tested: four polycultures, three bicultives, and four monocultures, in randomized blocks, with four replications. The productivity, total operating costs (TOC), gross and net revenue, rate of return, profitability index, and efficient land use were evaluated. The TOC values of intercropping were calculated with the prices of July 2015. In all the systems studied, the largest participation was referring to the cost hand of labor. The highest gross and net revenues were observed in the kale with lettuce in bicultive, the rate of return and profitability index was higher on lettuce in monoculture. Despite the increase in the TOCs of the intercropping in relation to the monocultures, the intercropping proved to be economically viable in terms of efficient land use, reaching values indicating a land-use efficiency of up to 50% more in polycultures and bicultives.

O cultivo de hortaliças de maneira mal planejada pode não alcançar os resultados econômicos esperados, pois é uma atividade que exige grande capacidade técnica e administrativa do produtor. O objetivo deste estudo foi avaliar a viabilidade econômica de cultivar couve, alface e cebolinha em consórcio. O experimento foi conduzido no Centro de Ciência e Tecnologia Agroalimentar da Universidade Federal de Campina Grande, no município de Pombal, PB, no período de junho de 2014 a julho de 2015. Foram testados onze tratamentos: quatro policultivos, três bicultivos e quatro monocultivos, em blocos casualizados, com quatro repetições. Foram avaliados: produtividade; custos operacionais totais (COT); receita bruta e líquida; taxa de retorno; índice de lucratividade e uso eficiente da terra (UET). Os valores de COT do consórcio foram calculados com os preços de julho de 2015. Em todos os sistemas estudados, a maior participação foi referente ao custo mão de obra. As maiores receita bruta e líquida foram observadas na couve com alface em bicultivo, a taxa de retorno e rentabilidade índice foram maiores na alface em monocultura. Apesar do aumento nos COT'S dos consórcios em relação às monoculturas, a consorciação mostrou-se economicamente viável em termos de uso eficiente da terra, alcançando valores que indicam uma eficiência no uso da terra de até 50% a mais em policultivos e bicultivos.

Cytogenotoxic effects of nitrogen on hydroponic lettuce / Efeitos citogenotóxicos do nitrogênio em alface hidropônica

Nitrogen accumulation in hydroponically-grown lettuce may pose a health risk to consumers. Thus, the objective of this study was to analyze different concentrations of nitrogen applications in hydroponic lettuce cultivation and their effect on toxicity, cytotoxicity and genotoxicity. A nutrient film technique (NFT) hydroponic system was used to grow the lettuce variety "Vanda." The treatments consisted of different concentrations of nitrogen (in the form of calcium nitrate) in Furlani solution (75, 100, 125 and 150%), a negative and a positive control. The following commercial characteristics were measured: plant fresh weight (PFW), root fresh weight (RFW), shoot fresh weight (SFW), shoot diameter (SD), root dry weight (RDW), shoot dry weight (SDW) and leaf nitrogen (LN). Cytogenotoxicity was indicated by toxicity, cytotoxicity and genotoxicity, which were in turn determined by root length, the mitotic index, chromosomal aberrations and the presence of micronuclei. The nitrogen concentrations used in this experiment did not cause phenotypic toxicity or cytotoxicity in lettuce roots. The most severe genotoxicity was observed at the 125% nitrogen concentration, which nevertheless did not affect commercial characteristics. Although nitrogen fertilization provides great benefits to agriculture, such as greater yields, indiscriminate use should be avoided since concentrations above recommended rates may induce genotoxicity.

O acúmulo de nitrogênio em alface cultivada hidroponicamente pode representar um risco à saúde dos consumidores. Assim, o objetivo deste estudo foi analisar diferentes concentrações de aplicações de nitrogênio no cultivo de alface hidropônica e seus efeitos na toxicidade, citotoxicidade e genotoxicidade. Em sistema hidropônico do tipo filme de nutrientes (NFT) foi usado para cultivar a variedade de alface "Vanda". Os tratamentos consistiram em diferentes concentrações de nitrogênio (na forma de nitrato de cálcio) na solução Furlani (75, 100, 125 e 150%), um controle negativo e um positivo. Foram medidas as seguintes características comerciais: peso fresco da planta (PFW), peso fresco da raiz (RFW), peso fresco da parte aérea (SFW), diâmetro da parte aérea (SD), peso seco da raiz (RDW), peso seco da parte aérea (SDW) e nitrogênio foliar (LN). A citogenotoxicidade foi indicada por toxicidade, citotoxicidade e genotoxicidade, que por sua vez foram determinadas pelo comprimento da raiz, índice mitótico, aberrações cromossômicas e presença de micronúcleos. As concentrações de nitrogênio utilizadas neste experimento não causaram toxicidade fenotípica ou citotoxicidade em raízes de alface. A genotoxicidade mais severa foi observada na concentração de nitrogênio de 125%, que, no entanto, não afetou as características comerciais. Embora a fertilização nitrogenada traga grandes benefícios à agricultura, como maiores rendimentos, o uso indiscriminado deve ser evitado, pois concentrações acima das taxas recomendadas podem induzir genotoxicidade.

Ocorrência de enteroparasitas em alfaces (Lactuca sativa) comercializadas em mercados municipais em Rio Branco, Acre / Occurrence of enteroparasitas in alfaces (Lactuca sativa) commercialized in municipal markets in Rio Branco, Acre / Presencia de enteroparasitos en lechuga (Lactuca sativa) comercializados en mercados municipales de Rio Branco, Acre

As hortaliças folhosas são ricas em vitaminas, sais minerais e fibras alimentares, proporcionando importantes benefícios para a saúde, o que acaba atraindo os consumidores, que costumam ingeri-las na forma crua, ficando expostos à contaminação por enteroparasitas. O objetivo do trabalho foi verificar a possível presença de enteroparasitas em alfaces (Lactuca sativa) comercializadas em três mercados municipais de Rio Branco, Acre. Foram analisadas 26 amostras de alfaces, onde as folhas das alfaces foram lavadas, em uma solução contendo 250 mL de água destilada e cinco gotas de detergente. O líquido foi filtrado em gazes de oito dobras e deixado sedimentar por 24 horas, à temperatura ambiente. Após o tempo decorrido o sobrenadante foi descartado, foram preparadas lâminas, que foram analisadas ao microscópio óptico. Os resultados obtidos neste estudo evidenciaram contaminação em 96,2% do total de amostras de alfaces analisadas. As principais estruturas parasitárias encontradas foram: Ascaridia sp., Eimeria sp., Entamoeba coli, Entamoeba histolytica, Ancylostoma sp., Strongyloides stercoralis, Enterobius vermicularis, Dipylidium caninum, Trichuris trichiura, Diphillobothrium latum e Balantidium sp. O enteroparasita mais abundante foi Ascaridia sp., tendo sido encontrados 164 (430) exemplares nas amostras, representando 38,14% do total de enteroparasitas encontrados. Ascaridia sp. foi também o enteroparasita mais frequente, sendo encontrada em 42,3% do total de amostras analisadas. Nesse contexto, a ingestão de alfaces cruas, provenientes dos mercados municipais estudados, pode representar risco potencial para os consumidores, caso elas não sejam higienizadas corretamente. Isto demonstra a importância de uma boa limpeza e desinfecção das folhas de alface antes do consumo.(AU)

Leafy vegetalizes are reich in vitaminas, mineral and. deitar febres, providing important health benefits, which ends up attracting consumers, who usually eat them in the raw form, being exposed to contamination by enteroparasites. The objective of the study was to verify the possible presence of enteroparasites in lettuce (Lactuca sativa) sold in three municipal markets in Rio Branco, Acre. 26 samples of lettuce were analyzed, where the lettuce leaves were washed, in a solution containing 250 mL of distilled water and. five drops of detergent. The liquid was filtered through eight-fold gauze and. left to settle for 24 hours at room temperature. After the elapsed time, the supernatant was discarded, slides were prepared and. analyzed under an optical microscope. The results obtained in this study showed contamination in 96.2% of the total samples of lettuce analyzed. The main parasitic structures found were: Ascaridia sp., Eimeria sp., Entamoeba coli, Entamoeba histolytica, Ancylostoma sp., Strongyloides stercoralis, Enterobius vermicularis, Dipylidium caninum, Trichuris trichiura, Diphillobothrium latum e Balantidium sp. The most abundant enteroparasite was Ascaridia sp., 164 (430) specimens were found in the samples, representing 38.14% of the total enteroparasites found. Ascaridia sp. was also the most common enteroparasite, was found in 42.3% of the total samples analyzed. In this context, ingestion of raw lettuce from the municipal markets studied may pose a potential risk to consumers if they are not properly sanitized. This demonstrates the importance of good cleaning and disinfection of lettuce leaves before consumption.(AU)

Las verduras de hoja son ricas en vitaminas, minerales y fibras dietéticas, aportando importantes beneficios para la salud, lo que acaba atrayendo a los consumidores, que suelen consumirlas crudas, quedando expuestas a la contaminación por enteroparásitos. El objetivo del estudio fue verificar la posible presencia de enteroparásitos en lechugas (Lactuca sativa) vendidas en tres mercados municipales de Rio Branco, Acre. Se analizaron 26 muestras de lechuga, donde se lavaron las hojas de lechuga, en una solución que contenía 250 mL de agua destilada y cinco gotas de detergente. El líquido se filtró a través de una gasa de ocho veces y se dejó reposar durante 24 horas a temperatura ambiente. Transcurrido el tiempo, se descartó el sobrenadante, se prepararon los portaobjetos y se analizaron al microscopio óptico. Los resultados obtenidos en este estudio mostraron contaminación en el 96,2% del total de muestras de lechuga analizadas. Las principales estructuras parasitarias encontradas fueron: Ascaridia sp., Eimeria sp., Entamoeba coli, Entamoeba histolytica, Ancylostoma sp., Strongyloides stercoralis, Enterobius vermicularis, Dipylidium caninum, Trichuris trichiura, Diphillobothrium latum y Balantidium sp. El enteroparásito más abundante fue Ascaridia sp., Se encontraron 164 (430) ejemplares en las muestras, lo que representa el 38,14% del total de enteroparásitos encontrados. Ascaridia sp. también fue el enteroparásito más frecuente, encontrándose en el 42,3% del total de muestras analizadas. En este contexto, la ingesta de lechuga cruda, de los mercados municipales estudiados, puede representar un riesgo potencial para los consumidores, si no se higienizan adecuadamente. Esto demuestra la importancia de una buena limpieza y desinfección de las hojas de lechuga antes del consumo.(AU)

Occurrence and diversity of ciliated protozoa in the most commonly consumed leafy greens in Brazil: a qualitative study / Ocorrência e diversidade de protozoários ciliados nos vegetais folhosos mais consumidos no Brasil: um estudo qualitativo

Ciliated protozoa are an ubiquitous group of eukaryotes that have been poorly reported on leafy greens. The present study aimed to verify the occurrence and diversity of ciliated protozoa in the leaves and roots of three of the most commonly consumed leafy greens in Brazil – lettuce, rocket and coriander. The vegetable samples were washed by manual agitation (3 minutes) in two different media (mineral water and Page ́s Amoeba Saline solution). After washing, the contents were incubated in Petri dishes and aliquots were removed for microscopic identification and in vivo observation. A total of 21 ciliated protozoa species were found, most of which were bacterivorous. Leafy greens have commonly been associated with foodborne outbreaks and ciliated protozoa, which although they are not a Public Health concern, can act as “Trojan Horses” harboring bacteria, viruses and other protozoa cysts and oocysts and can suggest a new route towards microbiological quality related to the food chain. This is the first report of ciliated protozoa on leafy greens consumed in Brazil.

Os protozoários ciliados constituem um grupo onipresente de eucariotos pouco relatados em vegetais folhosos. O presente estudo teve como objetivo verificar a ocorrência e a diversidade de protozoários ciliados nas folhas e raízes de três dos vegetais folhosos mais consumidos no Brasil - alface, rúcula e coentro. As amostras de vegetais foram lavadas por agitação manual (3 minutos) em dois meios diferentes (água mineral e Solução Salina para Ameba). Após a lavagem, o conteúdo foi incubado em placas de Petri e alíquotas foram retiradas para identificação microscópica e observação in vivo dos organismos. Um total de 21 espécies de protozoários ciliados foi encontrado, a maioria das quais era bacterívora. Tais hortaliças têm sido comumente associadas a surtos transmitidos por alimentos e os protozoários ciliados que, embora não sejam um problema de saúde pública, podem atuar como “Cavalos de Tróia” ao abrigar bactérias, vírus além de cistos e oocistos de protozoários o que pode sugerir uma nova rota para a avaliação da qualidade microbiológica relacionada à cadeia alimentar. Este é o primeiro relato de protozoários ciliados em folhas verdes consumidas no Brasil.